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  Discussion on Research Summary: Blood Test for Arthritis?
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DrO

Posted on Thursday, Jun 11, 2009 - 8:57 am:

One of the holy grails of arthritis research has been a blood biochemical marker that identifies subjects with arthritis and hopefully defines the type and severity. Well we are not there yet but in time might be a real possibility as demonstrated by the second paper presented below, however they recommend more work be done. For more on this see the article associated with this forum.
DrO

Res Vet Sci. 2009 Mar 17.
Relationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions.

Verwilghen D, Busoni V, Gangl M, Franck T, Lejeune JP, Vanderheyden L, Detilleux J, Grulke S, Deberg M, Henrotin Y, Serteyn D.

Department of Clinical Sciences, Equine Clinic, Faculty of Veterinary Medicine of Liege, Bld de Colonster 20 B41, Sart-Tilman B41, B-4000 Liege, Belgium.

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers of cartilage degradation and synovial inflammation. A specific radiological scoring and classification system was therefore developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochonDrOsis, degenerative joint disease and distal interphalangeal joint effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO(2) were used as blood markers. The combination of the blood parameters did not seem to correlate with the used scoring system. Coll2-1NO(2) levels however tended to increase with poorer radiological class and this could therefore potentially be a useful predictor of the osteoarticular status in the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with distal interphalangeal joint effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels.

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Equine Vet J. 2009 Jan;41(1):41-6.

Sandwich ELISA system for cartilage oligomeric matrix protein in equine synovial fluid and serum.

Yamanokuchi K, Tagami M, Nishimatsu E, Shimizu Y, Hirose Y, Komatsu K, Misumi K.

Department of Veterinary Clinical Science, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan.

REASONS FOR PERFORMING STUDY: Measurement of cartilage oligomeric matrix protein (COMP) in serum has potential for diagnosis of equine osteoarthritis (OA), but clinical use is currently limited by the lack of specificity of an inhibition ELISA as well as by baseline increases due to exercise. Improved methods for ELISA with increased antigen specificity and sensitivity are therefore required for reliable measurement. HYPOTHESIS: Measurement of the serum level of COMP by sandwich ELISA allows identification of horses with OA. METHODS: New monoclonal antibodies (mAbs) were elicited against equine cartilage COMP, their epitopes were determined and a sandwich ELISA was developed. The concentrations of COMP in synovial fluid (SF; n=100) and sera (n=100) from OA cases were measured by sandwich ELISA as well as by inhibition ELISA and compared with concentrations in normal joints (n=95) and horses (n=50). RESULTS: Immunoblots of enzymatically cleaved COMP showed that the new mAbs recognised different epitopes located on a 20 kDa fragment between K63 and K238 of the EGF-like repeats. Inhibition ELISA with any mAb detected significantly increased levels of COMP in OA SF compared with normal SF, whereas no significant difference was detected between serum levels of COMP in OA and normal horses. Conversely, sandwich ELISA with the combination of unlabelled 2A11 x biotinylated 11F10 mAbs detected a significant increase in COMP levels in both serum and SF from OA cases compared with levels in normal animals. CONCLUSIONS AND POTENTIAL RELEVANCE: Measurement of serum COMP with sandwich ELISA may be useful in identifying horses with OA.
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