Antibacterial Activity of Honey Samples

Honey, along with other good wound care practices, has resulted in healing of full skin thickness wounds that could not be closed. In some of the cases I have observed, infection was well controlled and healing occurred with manageable proud flesh and acceptable scar formation. I recommend its use under the supervision of a veterinarian, just to be careful.
DrO

Antibacterial Activity of Honey Samples from Ukraine
Vet Sci. 2020 Nov 20;7(4):E181.
Authors
Giovanni Cilia 1 , Filippo Fratini 1 , Matilde Marchi 1 , Simona Sagona 2 , Barbara Turchi 1 , Leonora Adamchuk 3 , Antonio Felicioli 1 , Miroslava Kačániová 4 5
Affiliations

1 Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 20159 Pisa, Italy.
2 Department of Pharmacy, University of Pisa, via Bonanno 6, 56126 Pisa, Italy.
3 Department of Horse-Breeding and Beekeeping, National University of Life and Environmental Sciences of Ukraine, Henerala Rodimtseva Str.19, 03041 Kyiv, Ukraine.
4 Department of Fruit Sciences, Viticulture and Enology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
5 Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszow, Cwiklinskiej 1, 35-601 Rzeszow, Poland.

PMID: 33233581
DOI: 10.3390/vetsci7040181

Abstract

The employment of natural substances such as beehive products with a preventive and therapeutic purpose has been a widespread custom since ancient times. In this investigation, the antibacterial activity of 41 honey samples from different Ukraine regions has been evaluated. For each honey, melissopalynological and physico-chemical analysis were performed in order to determine botanical origin, pH, glucose and fructose contents and free acidity. So, antibacterial activity against Staphylococcusaureus CCM 4223, Listeria monocytogenes ATCC 7644, Salmonella enterica serovar Typhimurium CCM 3807 and Escherichia coli ATCC 25922 was assessed through the determination of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values by the microdilutions method. The results show that the most susceptible bacterial strain was L. monocytogenes. Its growth was inhibited at a honey concentration ranging from 0.094 to 0.188 g/mL. The most resistant bacterial strain was S. aureus. As concerns MBC values, L. monocytogenes was the most susceptible bacteria, while S. aureus was the most resistant. Helianthus spp. honeys was the most effective against all tested bacterial strains, followed by Robinia spp. and multifloral honeys. Promising results for MIC tests have been found for Brassica spp.