Repair of OCD defects in the horse’s joints using mesenchymal cells

Quality repair of existing OCD lesions appears to be here. This report has found by selecting synovial stem cells, concentrating them, then placing them in the defect surgically that the original structure of the cartilage could be restored in 6 months. And amazing feat. Much still needs to done. Confirmation. Observing long term success. Repeating the work in different joints.
DrO

Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses
BMC Vet Res. 2022 Jan 22;18(1):53. doi: 10.1186/s12917-021-03126-y.
Authors
Daiki Murata 1 2 , Shingo Ishikawa 3 4 , Takafumi Sunaga 5 6 , Yasuo Saito 5 , Takeshi Sogawa 5 , Koichi Nakayama 7 , Seiji Hobo 3 , Takashi Hatazoe 3
Affiliations

1 Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan. daiki_net_official@yahoo.co.jp.
2 Department of Regenerative Medicine and Biomedical Engineering, Center for Regenerative Medicine Research, Faculty of Medicine, Saga University, Saga, Japan. daiki_net_official@yahoo.co.jp.
3 Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
4 Division of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Izumi-Sano, Japan.
5 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
6 Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
7 Department of Regenerative Medicine and Biomedical Engineering, Center for Regenerative Medicine Research, Faculty of Medicine, Saga University, Saga, Japan.

Abstract

Background: Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0 × 107 cells at passage 2 (P2).

Results: SM-MSCs were strongly positive for CD11a/CD18, CD44, and major histocompatibility complex (MHC) class I; moderately positive for CD90, CD105, and MHC class II; and negative for CD34 and CD45 on flow cytometry and differentiated into osteogenic, chondrogenic, and adipogenic lineages in the tri-lineage differentiation assay. After culturing SM-MSCs until P3, we prepared a construct (diameter, 6.3 mm; height, 5.0 mm) comprising approximately 1920 spheroids containing 3.0 × 104 cells each. This construct was confirmed to be positive for type I collagen and negative for type II collagen, Alcian blue, and Safranin-O upon histological analysis and was subsequently implanted into an osteochondral defect (diameter, 6.8 mm; depth, 5.0 mm) at the right femoral medial condyle. The contralateral (left femoral) defect served as the control. At 3 and 6 months after surgery, the radiolucent volume (RV, mm3) of the defects was calculated based on multiplanar reconstruction of computed tomography (CT) images. Magnetic resonance (MR) images were evaluated using a modified two-dimensional MR observation of cartilage repair tissue (MOCART) grading system, while macroscopic (gross) and microscopic histological characteristics were scored according to the International Cartilage Repair Society (ICRS) scale. Compared to the control sites, the implanted defects showed lower RV percentages, better total MOCART scores, higher average gross scores, and higher average histological scores.

Conclusions: Implantation of a scaffold-free 3D-construct of SM-MSCs into an osteochondral defect could regenerate the original structure of the cartilage and subchondral bone over 6 months post-surgery in horses, indicating the potential of this technique in treating equine subchondral bone cysts.

Keywords: Horse; Mesenchymal stem cell; Regeneration; Subchondral bone cyst; Synovial membrane.